Zvigadzirwa
Hepps; Cas No.: 16052-06
Synonyms. acid; 3- [4- (2-hydroxytl) -1-pombyuny) propaneshfonic acid, 4- Asidhi; n- (2-hydroxytHyl) piperazine-n '- (((((((pombixyryl) -1-pladroxylf. 240-198-8; Brn 385; 8JN5PQw99c; 4- (Pepa) Sulfonic Acid; MFCD00006160; acid; EP1; N-2-HydroxyHpylponezine-n'-Propaneshfonic acid; Unii-8JN5PQW9999; Chebi: 42298; Epps,> = 99.5% (titation); hy-d0873; c9-h20-n2-o4-s; bbl02267163; epps 99%; AC-24630; AS-69273; EPS, Bioxtra,> acid; n- (hydroxytHyl) piperazine-n'-propane sulfonic acid; n-2-hydroxytlponelpone-n-3-provanesulfonic asidhi; q1563525; acid; n- (2-hydroxythyl) piperazine-n'- (((-hydroxylfonic acid); Bioperformance Certified, Cell Clement,> = 99.5% (titration); 3- [4- (2-((4-((-hydroxythusl) piperazin-1-yl] propylsulfonyloxonium
Chemical chivakwa che hepps
● Chitarisiko / Ruvara: White Crystalline poda
● Rolling Pfungwa: 237-239 ° C (Lit.)
● Refractive Index: 1.537
● PKA: 8.0 (na25 ℃)
● PSA:89.46000
● Density: 1.285 G / CM3
● logp: -0.16920
● Kuchengetedza temp.Iksore kuRT.
● Solubility.:h2o: 1 m pa 20 ° C, yakajeka, isina ruvara
● Solub Solubility.:Selble mumvura. 25.2 g / l (20 ° C)
● XLOGP3: -3.6
● Hydrogen Bond anopa kuverenga: 2
● Hydrogen Bond Anogamuchira Kuverenga: 6
● Kutyisa Bond Count: 6
● Zvakakodzera: 252.11437830
● Anorema Atomu Kuverenga: 16
● kuomarara: 280
Ruzivo rwekufa
● pikicha (s):
Xi
● Hazard Codes: Xi
● Zvirevo: 36/37 / 38-22
● Zvirevo Zvekuchengetedza: 24/12-36-26
Zvinoshanda
Makirasi emakemikari:Nitrogen Compounds -> Pepairazine
Canonical kunyemwerera:C1CN (CCN1CCCS (= O) (= O) O) CCO
Inoshandisa:Epps chinhu chinowanzoshandiswa zvakanaka? S buffer. Iyo inowanzoshandiswa sekuparadzaniswa muUltrathin isoelectric yekutarisa geli uye inosimudzira resolution ye phoshoglucomut. Iyo inoshandiswa seyemumiriri anodhira mu biology uye biochemistry.
Tsanangudzo yeruzivo
Epps inomirira n- (2-hydroxytl) piperazine-n '- ((propaneneulfonic acid). Iyo iziterionic buffering mumiririri anowanzoshandiswa muBiiological ne Biochemical researchs.Here dzimwe nguva dzinokosha nezve EPS:
Kubuditsa zvinhu:Epps chiitiko chinoshanda chinobatsira chinobatsira chengetedza nguva dzose pH mukuedza kwekuedza. Iyo ine kukosha kwePI 8.09, iyo inoita kuti ive yakakodzera yekubuditsa muPH Range ye 7.0-9.0.
Kugadzikana:Epps inoratidzira kugadzikana kwakanaka pamusoro pehuremu hwakawanda hwekupisa uye kusanyanyokanganiswa nekudziya kwekudziya uchienzaniswa nevamwe vamiriri veTRIFT uye phosphate buffers. Kugadzikana uku kunoita kuti zviite kuti zviite kushandiswa muBiiochemical uye muyedzo wekuongorora izvo zvinoda zvinodzora ph mamiriro.
Biological application:Epp Yayo inokwanisa kugona uye kugadzikana pamhyasi ph inoita kuti ive yakakosha mune izvi zvinoshandiswa.
Tsika Tsika:Epps inogona zvakare kushandiswa seyemumiriri mudenga mutsika tsika. Iyo inobatsira kuchengetedza nzvimbo yakagadzikana pP inokodzera kuwanda uye kuchengetedza mamwe marudzi echitokisi.
Ion uye kuwirirana:Epps ndeye zwitterionic compand, zvinoreva kuti ine zvese zvakanaka uye zvisina kunaka mhosva. Iyi ficha inobvumira kuti iite zvinobudirira buffer ese ari acidic uye yekutanga mhinduro. Izvo zvinowirirana nemamolekora akasiyana siyana ebonde uye anozivikanwa kuve nekukanganiswa kushoma ne enzymatic maitiro.
Paunenge uchishandisa Epps, zvakakosha kutevedzera iyo yakakurudzirwa yekusangana uye nhungamiro yakapihwa nemugadziri. Pamusoro pezvo, gara uchichenjerera uye uteedzere kurongeka kwakakodzera protocol paunenge uchibata chero makemikari kana kubhowa mumiririri.
Application
Hepps (N- (2-HydroxyTHETL) Piperazine-N'-Propaneshfonic acid) iri zwitterionic buffer inowanzo shandiswa mukutsvaga muBiological neBiochemical. Zvimwe zvezvishandiso zvaro zvinosanganisira:
Tsika Tsika: HePP inoshandiswa seyekubaya mumiririri wezvetsika tsika kuchengetedza ph uye kupa nharaunda yakakodzera yekukura kwesero uye kuwedzera. Iyo inobatsira kusimbisa iyo pH mukati meiyo phepiti renji uye dzivisa chero mhedzisiro yakaipa pamasero maitiro.
Enzyme assays:Hepps inowanzoshandiswa sebuffer muEnzyme assays kuti uwedzere iyo PH mamiriro anodiwa kune yakakwira enzyme chiitiko. Kugona kwayo kwekugona kunobatsira kuchengetedza yakagadzikana pH, ichisvika kune yakakwana uye yakavimbika enzyme zviyero.
Protein Kunatswa:Hepps inogona kushandiswa seyakagadzirwa muProtein kucheneswa kwekunatswa. Iyo inobatsira kuchengetedza kugadzikana uye kuita zvemapuroteni panguva yekuchenesa matanho, senge column chromatography kana protein kuchenesa uchishandisa zvinyorwa zvehukama.
Electrophoresis:Hepps dzimwe nguva inoshandiswa seyakagadzirwa mune gel electrophoresis yekuparadzanisa uye kuongororwa kwemapuroteni uye accleic acids. Its ph buffering kugona inobatsira kuchengetedza yakagadzikana pH panguva yeElectrophoretic kumhanya, kubatsira mukuparadzaniswa kwakaringana kwemamorekuru.
Molecular Biology Matteni:Hepps inogona kushandiswa seyakasarudzika mumenderekedzo yemifananidzo yebialogy, inosanganisira PCR (Polymerase Chain Reaction), DNA kutsvaira, uye enzymatic maitiro. Kugona kwayo kwekugona kunobatsira kune yakakwana ph mamiriro ekubudirira uye anoshanda maitiro.
Zvidzidzo zvinosanganisira mapuroteni ememberi:Hepps yakanyatsokodzera kudzidza membrane mapuroteni nekuda kweiyo yakadzika membrane-penetirate zvivakwa. Iyo inowanzoshandiswa seye buffer mukuedza inosanganisira iyo inoshanda chimiro kana kugovera kwemapuroteni emambrane.
Izvo zvakakosha kuti uzive kuti iyo yekuisa pfungwa uye iyo pH ye hepps inogona kusiyana zvichienderana nekunyorwa kwayo kana mamiriro ekuedza. Naizvozvo, zvinokurudzirwa kuti ubvunze mabhuku emabhuku kana ekupa mari yekushandiswa kwakakodzera uye kusunganidzwa kweepps muedzo yako.
