Products
HEPES sodium munyu
Mashoko anoreva zvakafananaHEPES sodium munyu;75277-39-3;HEPES hemisodium munyu;103404-87-1;Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate;sodium 2-[4-(2-hydroxyethyl) )piperazin-1-yl]ethanesulfonate;HEPES (sodium);C8H17N2NaO4S;4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid hemisodium munyu;1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, monosodium munyu;4-(2-Hydroxyethyl)piperazine-1- ethanesulfonic acid sodium munyu; Sodium 4-(2-hydroxyethyl)piperazin-1-ylethanesulphonate;UNII-Z9FTO91O8A;Z9FTO91O8A;sodium;2--[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate;DTXSID4044458;CHEBI;EINECS55;CHEBI:4675; 278-169-7;1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, sodium munyu (1:1);N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid,sodium munyu;HEPES sodium munyu, > = 99.5% (titration); sodium hepes;N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) sodium munyu;MFCD00036463;HEPES, sodium munyu;HEPES sodium munyu, 98%;C8H18N2O4S.Na;SCHEMBL229142;WAS-14;CHEMBL3187284;DTXCID2024458;RDZTWEVXRGYCFV-UHFFFAOYSA-M;C8-H18-N2-OPE-S munyu.Na; (1M);Tox21_302155;HB5187;N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonicacid)hemisodiumsalt;HEPES sodium munyu, biochemical grade;AKOS015897769;AKOS015912229;AKOS015964204;NCGC00255791-01;CAS-75277-39-3;HY-108535;CS-0029103;FT-0610308618; N'-hydroxyethyl-N-piperazineethanesulfonate;HEPES sodium munyu, BioXtra, >=99.0% (titration);HEPES sodium munyu, Vetec(TM) reagent giredhi, 96%;W-104397;Q27120698;Sodium2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate;4-(2-Hydroxyethyl) -1-piperazineethanesulfonic acid sodium munyu;4-(2-hydroxyethyl)- 1-piperazineethanesulfonic acid, monosodium munyu;N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)sodiumsalt;N-2-Hydroxyethylpiperazine-N'-2-ethanesulphonic acid hemisodium munyu;HEPES sodium munyu, BioPerformance Certified, inokodzera sero tsika, > =99.5%
Chemical Property yeHEPES Sodium Munyu
● Chitarisiko/Ruvara:upfu chena
● Vapor Pressure: 0Pa pa 25 ℃
● Panonyungudika:234 °C
● PKA:7.5(pa25℃)
● PSA:92.29000
● Density:1.504[pa20℃]
● LogP:-0.90190
● Kuchengetedza Kwekushisa.:Chengeta paRT.
● Sensitive.:Hygroscopic
● Solubility.:H2O: 1 M pa20 °C, yakajeka, isina ruvara
● Kunyungudika kwemvura.:Inonyungudika mumvura.
● Hydrogen Bond Donor Count:1
● Hydrogen Bond Acceptor Count:6
● Inotenderera Bond Count:5
● Chaiyo Misa:260.08067248
● Nhamba Yeatomu Inorema:16
● Kuoma kunzwisisa:272
Safty Information
● Pictogram(s):
● Mitemo Yenjodzi:
● Mashoko Okuchengetedza: 22-24/25
Useful
Makemikari Makirasi:Zvimwe Zvishandiso -> Biological Buffers
Canonical SMILES:C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+]
Zvishandiso:HEPES sodium munyu mhinduro inokodzera sero tsika zvidzidzo. Inogona kushandiswa mukugadzira buffer mhinduro yeHEPES. Inogona kushandiswa sebuffer panguva ye phosphorylation assays mune permeabilized fibroblasts. Inogona kushandiswa kugadzira yakaoma dialysis buffer, inoshandiswa mukutsunga yemahara thyroxin mu serum ne radioimmunoassay. HEPES sodium munyu mhinduro yakakodzera kushandiswa muzvidzidzo zvetsika. Inogona kushandiswa mukugadzira buffer mhinduro yeHEPES. Inogona kushandiswa sebuffer panguva ye phosphorylation assays mune permeabilized fibroblasts. Inogona kushandiswa kugadzira yakaoma dialysis buffer, inoshandiswa mukutsunga yemahara thyroxin mu serum ne radioimmunoassay.
Detailed Sumo
HEPES sodium munyu, inozivikanwawo se 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid sodium munyu, inowanzoshandiswa fomu yeHEPES. Iyo zwitterionic organic komisheni inoshanda senge inodzivirira mubiological uye biochemical research.
HEPES sodium munyu inowanzo shandiswa musero tsika midhiya uye biological buffers nekuda kwekugona kwayo kuchengetedza yakagadzikana pH siyana yakapoteredza mamiriro emuviri (pH 7.2 - 7.6). Inonyungudika zvakanyanya mumvura uye ine huturu hushoma, zvichiita kuti ive yakakodzera kune dzakasiyana siyana.
Iyo sodium munyu fomu yeHEPES inofarirwa mune akawanda maapplication sezvo ichiwedzera kunyungudika uye kugadzikana, zvichienzaniswa neiyo yemahara asidhi fomu. Inowanikwa mune zvekutengeserana sechena crystalline powder inogona kunyungudutswa nyore nyore mumvura kuti igadzirire kushanda mhinduro.
Vatsvagiri vanowanzo shandisa HEPES sodium munyu mumamolekyuli biology matekiniki, sero tsika, enzyme assays, kucheneswa kweprotein, uye kumwe kuedza kwebiochemical uko pH kudzora kwakakosha. Kubata kwayo simba uye kuenderana neiyo biological masisitimu inoita kuti ive sarudzo yakakurumbira yekuchengetedza mamiriro akakwana echiito uye kugadzikana kweakasiyana biological mamorekuru.
Usati washandisa HEPES sodium munyu mune chero chaiyo application, zvakakosha kuti utarise zvinyorwa, bvunza kurudziro yevatengesi, uye kukwidziridza iyo yekumisikidza uye pH renji zvichienderana nezvinodiwa zvekuedza.
Application
HEPES sodium munyu inowana kushandiswa kwakapararira munzvimbo dzakasiyana dzebiological uye biochemical yekutsvagisa. Zvimwe zvekushandisa kwayo kukuru zvinosanganisira:
Cell Culture:HEPES sodium munyu inowanzowedzerwa kune cell tsika midhiya kuchengetedza yakagadzikana pH mukati meiyo physiological renji uye inopa nharaunda yakakodzera kuti maseru akure uye awedzere.
Buffering Agent:HEPES sodium munyu inogara ichishandiswa seyakavharisa mubiological buffers uye mhinduro, kusanganisira enzyme assays, protein purification, uye molecular biology maitiro. Inobatsira kuchengetedza inogara pH nekuramba shanduko mu acidity kana alkalinity.
Electrophoresis: HEPES sodium munyu inoshandiswa sebuffer mu gel electrophoresis kuchengetedza yakagadzikana pH panguva yekuparadzaniswa kwe nucleic acids kana mapuroteni. Iyo inodzivirira shanduko mupH iyo inogona kukanganisa mafambiro uye kupatsanurwa kwemaitiro emamorekuru mugel.
Protein Stability: HEPES sodium munyu inowedzerwa kune mapuroteni mhinduro kudzikamisa chimiro chavo uye kuchengetedza basa ravo. Inobatsira kuchengetedza yakakwana pH nharaunda inodiwa kuti protein igadzikane uye kushanda.
Enzyme Chiitiko: HEPES sodium munyu inoshandiswa sechivharo mukuita enzymatic kuchengetedza iyo inodiwa pH yekuita yakakwana enzyme. Inobatsira kuve nechokwadi chekuti ma enzymes anoshanda pakunyatso shanda uye nekurongeka.
Live Cell Imaging:HEPES sodium munyu inowanzobatanidzwa mune yekufungidzira midhiya yehupenyu hwesero imaging experiments. Iyo buffering kugona kwayo inobatsira kuchengetedza iyo inodiwa pH uye inodzivirira shanduko inogona kukanganisa kushanda uye fluorescence yemaseru.
Molecular Biology Techniques: HEPES sodium munyu inoshandiswa zvakanyanya mumhando dzakasiyana siyana dzebhaibheri biology, kusanganisira DNA kana RNA isolation, PCR, DNA sequencing, uye protein analysis. Iyo buffering kugona kwayo inobvumira mhedzisiro uye yakavimbika mhedzisiro panguva idzi maitiro.
Zvakakosha kuziva kuti mashandisirwo chaiwo uye kuiswa kweHEPES munyu wesodium kunogona kusiyana zvichienderana nezvinodiwa zvekuedzwa uye biological system iri pasi pekudzidza. Naizvozvo, zvinokurudzirwa kubvunza mabhuku uye kurudziro yevatengesi kuti ishandiswe zvakakwana.
