Zvigadzirwa
Hemes; Cas No.: 7365-45-9
Synonyms. acid); n- (2-hydroxythyl) piperazine-n'-2-etdroxyfonic acid; nsc 166663; n '- (((((((26-hydroxyfone) piperazine-n- (2-ethanesulfonic acid); n'-2-hydroxylpipirazine-n-2-ethanesulfonic acid; tvz 7; Thandroxetyl
Zvivakwa zvemakemikari zvehipes
● Chitarisiko / Ruvara: White Crystalline poda
● Vapor Dzvinyiriri: 0pa pa25 ℃
● Rolling Pfungwa: 234-238 ° C
● Refractive Index: N20 / D 1.3939
● Boling Point: 408 ℃ [at 101 325 PA]
● PKA: 7.5 (na25 ℃)
● PSA:89.46000
● Density: 1.325 g / cm3
● logp: -0.55930
● Kuchengetedza Temp.:26 OLOC
● Solubility.:h2o: 1 m pa 20 ° C, yakajeka, isina ruvara
● Solublity yemvura.:soluble
● XLOGP3: -4.1
● Hydrogen Bond anopa kuverenga: 2
● Hydrogen Bond Anogamuchira Kuverenga: 5
● Kutyisa Bond Kuverenga: 4
● Nhoroondo Yechokwadi: 238.09872823
● Anorema Atomu Kuverenga: 15
● kuomarara: 254
Ruzivo rwekufa
● pikicha (s):
Xi
● Hazard Codes: Xi
● Zvirevo: 36/37/38
● Zvirevo Zvekuchengetedza: 24 / 25-22-36-26
Zvinoshanda
Canonical kunyemwerera:C1CN (CC [NH +] 1CCS (= O) (= O) [O -]) CCO
Tsanangudzo:Hopes anotsanangurwa seimwe yeakanakisa-yakanaka-chinangwa buffers inowanikwa yekutsvaga kwezvinhu zvakanaka. PaBiological pH, molecule ndeye zwitterionic, uye inoshanda sebuffer kuPH 6.8 kusvika 8.2 (pka 7.55). Izvo zvinowanzo shandiswa muchitokisi tsika pakusangana pakati pe5mm kusvika pamakumi matatu mm. Hepes yakashandiswa mune dzakasiyana siyana dzekushandisa, kusanganisira tsika yematehwe. Zvinowanzoshandiswa kubaya cell cell tsika midhiya mumhepo. Hemes anowana kushandisa kwayo muVitro kuyedza paMG.
Inoshandisa:Zvisiri zvepfu. Iyo inoshandiswa se hydrogen ion buffer, iyo inogona kudzora iyo nguva dzose ph huwandu kwenguva yakareba. Iyo yekuvhara ndeye 10-50mol / l. Kazhinji, 20mmol / lhepes mumhinduro yemutengo inogona kuwana buffer kugona. Biological buffer. Hupes anowanzo buda kune sainzi yezvibayiro, inonyanya kushandiswa mutasero tsika kuchengetedza phimalic ph. Izvo zvinogadzirisa chinhu chinodhiraivha, chinoshandiswa mukugadzirira kweBuffers. Zvinotsanangurwa seimwe yeakanakisa-mese-chinangwa buffers
Tsanangudzo yeruzivo
HopesInomiririra 2- ((((hydroxythewl) piperazin-1-yl) ethanesulfonic acid. Iyo yekudhara mumiririri inowanzo shandiswa muBiiological neBiochemical research. Hupes inobatsira kuchengetedza iyo yakagadzikana pH muchitokisi yetsika, sezvo inokwanisa kugamuchira uye kupa protons, nekudaro yekudzora pr shanduko pH. Maitiro ayo emakemikari uye zvivakwa zvinoita kuti zviite zvakanyanya mukuchengetedza nguva dzose uye yakakwana pH kune akawanda ebiological uye enzymatic maitiro. Hemes anowanzoshandiswa muchitokisi tsika, protein kucheneswa, uye mendeko biology maitiro.
Application
Hemene ane akati wandei maapplication muminda yakasiyana-siyana yekutsvaga. Zvimwe zvezvimwe zvinoshandiswa zvehiya ndezvekuti:
Tsika Tsika: Hemes anowanzoshandiswa sechinhu muzvinhu zvetsika tsika kuchengetedza nharaunda yakagadzikana. Iyo inobatsira kudzora shanduko pH inokonzerwa ne metabolic zviitiko uye kuunganidzwa kwezvinhu zvetsika mumasero tsika.
Enzyme assays:Ashipes anowanzo shandiswa seyemumiriri mu enzyme assays kuchengetedza phwo nguva dzose ph, iyo yakakosha kune yakakodzera kushanda kwe enzymes. Iyo inobatsira kuve nechokwadi chakasimba uye chinobereka mhedzisiro.
Electrophoresis:Hopes anoshandiswa seyekuchengetera mumiriri muElectrophoresis maitiro kunge Polwandagiramu Electrophoresis (Peji) uye Agarose gel Electrophoresis. Iyo inobatsira kuchengetedza iyo yaunoda pH Range kuti ive yakakwana kupatsanurwa uye kuongororwa macromolecules akadai semapuroteni uye nucleic acids.
Protein Kunatswa:Hemes dzimwe nguva inosanganisirwa mune protein kucheneswa yekuchenesa kuchengetedza iyo inodiwa pH panguva dzakasiyana dzekuchenesa maitiro ekuzvinatsa. Iyo inobatsira kuchengetedza kugadzikana uye kuita kwemapuroteni panguva yekuzvinatsa.
Molecular Biology Matteni:Hopes inogona kushandiswa mumhando dzakasiyana-siyana dzezvinyorwa zvebuology, dzakadai sePCR (Polymerase Chain Reaction) uye DNA Kukutsikiswa, kuchengetedza yakagadzikana pH. Iyo inobatsira gadzirisa mashandiro enzymes uye anovimbisa mibairo yakakwana uye yakavimbika.
Izvo zvakakosha kuti uzive kuti iyo chaiyo yekuvhara yeHerpes uye application yayo inogona kusiyana zvichienderana nemamiriro ekuedza uye zvinodiwa.
